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Image Search Results
Journal: Journal of Food and Drug Analysis
Article Title: Biological and clinical significance of the AGE-RAGE axis in the aggressiveness and prognosis of prostate cancer
doi: 10.38212/2224-6614.3475
Figure Lengend Snippet: AGEs promote cell proliferation and decrease sensitivity to chemodrugs in PCa cells. (A) Androgen-dependent LNCaP and 22rv1, and (B) androgen-independent DU145 and PC3 cells were treated for 48 h with five types of AGEs-BSA (10–500 μg/mL) derived from different glycated agents. Cell viability was measured using the MTS assay and expressed as a relative percentage of the BSA control. GA-derived and GOA-derived AGEs increased PCa cell proliferation, whereas the other AGEs failed to enhance cell proliferation. (C) Treatment with anti-RAGE antibodies inhibited GA-derived AGEs-induced cell proliferation. PCa cells were co-treated with GA-derived AGE and anti-RAGE antibodies (10 μg/mL) for 48 h. Treatment of (D) AGEs-BSA (500 μg/mL) and (E) AGE compounds increased resistance to chemodrug toxicity in PCa cells. The cells were co-treated with AGEs and DTX (7.2 μM) for 24 h, and the cell viability was measured. GA-derived and GOA-derived AGEs, CEL, and CML increased PCa cell survival in response to the DTX challenge. (F) sRAGE counteracted CML-mediated chemoresistance and rendered PCa cells more sensitive to DTX toxicity. The cells were preincubated with sRAGE for 24 h, after which DTX was added and incubated for another 24 h. Data shown represents the means ± SD from three independent experiments with 6–8 replicates each. *P < 0.05 when compared with the BSA or vehicle control. DTX, docetaxel; anti-RAGE ab, anti-RAGE antibody; sRAGE, soluble RAGE.
Article Snippet: The
Techniques: Derivative Assay, MTS Assay, Control, Incubation